You need to use Sender as AsyncFileUpload.
Ref:
<html xmlns="http://www.w3.org/1999/xhtml">
<head runat="server">
    <style type="text/css">
        body
        {
            font-family: Arial;
            font-size: 10pt;
        }
    </style>
    <title>AsyncFileUpload Example</title>
    <script type="text/javascript">
        function uploadComplete(sender) {
            $get("<%=lblMesg.ClientID%>").style.color = "green";
            $get("<%=lblMesg.ClientID%>").innerHTML = "File Uploaded Successfully";
        }
        function uploadError(sender) {
            $get("<%=lblMesg.ClientID%>").style.color = "red";
            $get("<%=lblMesg.ClientID%>").innerHTML = "File upload failed.";
        }
    </script>
</head>
<body>
    <form id="form1" runat="server">
    <asp:ScriptManager ID="ScriptManager1" runat="server">
    </asp:ScriptManager>
    <asp:GridView ID="GridView1" runat="server" AutoGenerateColumns="false">
        <Columns>
            <asp:BoundField DataField="Id" HeaderText="Id" />
            <asp:TemplateField HeaderText="Upload">
                <ItemTemplate>
                    <cc1:AsyncFileUpload OnClientUploadError="uploadError" OnClientUploadComplete="uploadComplete"
                        runat="server" ID="AsyncFileUpload1" Width="400px" UploaderStyle="Modern" CompleteBackColor="White"
                        UploadingBackColor="#CCFFFF" ThrobberID="imgLoader" OnUploadedComplete="FileUploadComplete" />
                </ItemTemplate>
            </asp:TemplateField>
        </Columns>
    </asp:GridView>
    <asp:Image ID="imgLoader" runat="server" ImageUrl="~/images/loader.gif" />
    <br />
    <asp:Label ID="lblMesg" runat="server" Text=""></asp:Label>
    </form>
</body>
</html>
Namespace
using System.Xml.Linq;
using AjaxControlToolkit;
C#
protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        DataTable dt = new DataTable();
        dt.Columns.AddRange(new DataColumn[1] { new DataColumn("Id", typeof(int)) });
        dt.Rows.Add(1);
        dt.Rows.Add(2);
        GridView1.DataSource = dt;
        GridView1.DataBind();
    }
}
protected void FileUploadComplete(object sender, EventArgs e)
{
    string filename = System.IO.Path.GetFileName((sender as AsyncFileUpload).FileName);
    (sender as AsyncFileUpload).SaveAs(Server.MapPath("Uploads/") + filename);
}
Screenshot
