Hi @atul,
Try the following sample.
Html Code
   <asp:GridView ID="gvCustomers" runat="server" AutoGenerateColumns="false">
            <Columns>
                <asp:TemplateField Visible="false">
                    <ItemTemplate>
                        <asp:HiddenField ID="hfCustomerId" runat="server" Value='<%#Eval("Id") %>' />
                    </ItemTemplate>
                </asp:TemplateField>
                <asp:BoundField DataField="Name" HeaderText="Customer Name" />
            </Columns>
        </asp:GridView>
Code:
protected void Page_Load(object sender, EventArgs e)
        {
            if (!this.IsPostBack)
            {
                this.gvCustomers.DataSource = this.GetData();
                this.gvCustomers.DataBind();
                this.AccessGridData();
            }
        }
        private void AccessGridData()
        {
            int customerId = 0;
            string customerName = string.Empty;
            foreach (GridViewRow row in this.gvCustomers.Rows)
            {
                if (row.RowType == DataControlRowType.DataRow)
                {
                    customerId = int.Parse((row.FindControl("hfCustomerId") as HiddenField).Value);
                    customerName = row.Cells[1].Text;
                }
            }
        }
        private DataTable GetData()
        {
            DataTable dt = new DataTable();
            dt.Columns.AddRange(new DataColumn[2] { new DataColumn("Id"), new DataColumn("Name") });
            dt.Rows.Add(1, "Ashutosh");
            dt.Rows.Add(2, "Ram Krishna");
            dt.Rows.Add(3, "Saajid");
            dt.Rows.Add(4, "Shailesh");
            return dt;
        }
Namespaces:
using System;
using System.Web.UI.WebControls;
using System.Data;