Hi  vishalkal,
Please refer below sample.
According to your requiremet modified code taking reference to the below code.
Namespaces
C#
using System.Data;
using System.Windows.Forms;
using System.Globalization;
VB.Net
Imports System.Globalization
Code
C#
private void BindGrid()
{
    dataGridView1.AutoGenerateColumns = false;
    DataTable dt = new DataTable();
    dt.Columns.AddRange(new DataColumn[] { new DataColumn("Id", typeof(string)), 
                                    new DataColumn("Ammount", typeof(int)) ,
                                    new DataColumn("Item", typeof(string))});
    dt.Rows.Add("1", 11, "Mango");
    dt.Rows.Add("2", 20, "Banana");
    dt.Rows.Add("3", 32, "Grapes");
    dataGridView1.AutoGenerateColumns = false;
    dataGridView1.ColumnCount = 3;
    dataGridView1.Columns[0].Name = "Id";
    dataGridView1.Columns[0].HeaderText = "Id";
    dataGridView1.Columns[0].DataPropertyName = "Id";
    dataGridView1.Columns[1].HeaderText = "Ammount";
    dataGridView1.Columns[1].Name = "Ammount";
    dataGridView1.Columns[1].DataPropertyName = "Ammount";
    dataGridView1.Columns[2].Name = "Item";
    dataGridView1.Columns[2].HeaderText = "Item";
    dataGridView1.Columns[2].DataPropertyName = "Item";
    dataGridView1.DataSource = dt;
}
private void dataGridView1_CellFormatting(object sender, DataGridViewCellFormattingEventArgs e)
{
    if (e.ColumnIndex == 1 && e.RowIndex != this.dataGridView1.NewRowIndex)
    {
        int a = Convert.ToInt16(e.Value);
        e.Value = a.ToString("C2", CultureInfo.CurrentCulture);
    }
}
private void Form1_Load(object sender, EventArgs e)
{
    this.BindGrid();
}
VB.Net
Private Sub BindGrid()
    dataGridView1.AutoGenerateColumns = False
    Dim dt As DataTable = New DataTable()
    dt.Columns.AddRange(New DataColumn() {New DataColumn("Id", GetType(String)), New DataColumn("Ammount", GetType(Integer)), New DataColumn("Item", GetType(String))})
    dt.Rows.Add("1", 11, "Mango")
    dt.Rows.Add("2", 20, "Banana")
    dt.Rows.Add("3", 32, "Grapes")
    dataGridView1.AutoGenerateColumns = False
    dataGridView1.ColumnCount = 3
    dataGridView1.Columns(0).Name = "Id"
    dataGridView1.Columns(0).HeaderText = "Id"
    dataGridView1.Columns(0).DataPropertyName = "Id"
    dataGridView1.Columns(1).HeaderText = "Ammount"
    dataGridView1.Columns(1).Name = "Ammount"
    dataGridView1.Columns(1).DataPropertyName = "Ammount"
    dataGridView1.Columns(2).Name = "Item"
    dataGridView1.Columns(2).HeaderText = "Item"
    dataGridView1.Columns(2).DataPropertyName = "Item"
    dataGridView1.DataSource = dt
End Sub
Private Sub dataGridView1_CellFormatting(ByVal sender As Object, ByVal e As DataGridViewCellFormattingEventArgs)
    If e.ColumnIndex = 1 AndAlso e.RowIndex <> Me.dataGridView1.NewRowIndex Then
        Dim a As Integer = Convert.ToInt16(e.Value)
        e.Value = a.ToString("C2", CultureInfo.CurrentCulture)
    End If
End Sub
Private Sub Form1_Load(ByVal sender As Object, ByVal e As EventArgs)
    Me.BindGrid()
End Sub
Screenshot
