Yes you need to create the Temp folder.

Else you need to add the below code for Create folder if not present.

C#

//If Directory not present, create it.
if (!Directory.Exists(Server.MapPath("~/Temp/")))
{
    Directory.CreateDirectory(Server.MapPath("~/Temp/"));
}

VB.Net

'If Directory not present, create it.
If Not Directory.Exists(Server.MapPath("~/Temp/")) Then
    Directory.CreateDirectory(Server.MapPath("~/Temp/"))
End If