Hi @tanuherma

You can disable it using javascript

Refer this code

 Javascript

<script type="text/javascript">
        function Disable() {
            var Grid = document.getElementById('<%=GridView1.ClientID%>');
            var NoOfColumns = 3 // Set No. of Columns in GridView
            for (var i = 0; i < NoOfColumns; i++) {
                Grid.rows[1].cells[i].children[0].disabled = true;
            }
            return false;
        }
    </script>

HTML

<asp:Button ID="Button1" runat="server" Text="Button" OnClientClick="return Disable()"  />