Hi  iammann,
I have created sample that full fill your requirement.
HTML
<div>
    <asp:Calendar ID="Calendar1" OnDayRender=" Calendar1DayRender" runat="server"></asp:Calendar>
    <br />
    <b><span style="color: Red">Red</span></b><span> - Booked</span>
</div>
Code
protected DataSet dsDates;
protected void Page_Load(object sender, EventArgs e)
{
    if (!IsPostBack)
    {
        Calendar1.VisibleDate = DateTime.Today;
    }
    GetDates();
}
//Get Dates from DataBase.
protected void GetDates()
{
    DataTable dt = new DataTable();
    dt.Columns.AddRange(new DataColumn[1] { new DataColumn("BookedDate", typeof(DateTime)) });
    dt.Rows.Add(DateTime.Now);
    dt.Rows.Add(DateTime.Now.AddDays(5));
    dt.Rows.Add(DateTime.Now.AddDays(-5));
    dt.Rows.Add(DateTime.Now.AddDays(30));
    dt.Rows.Add(DateTime.Now.AddDays(-30));
    dt.Rows.Add(DateTime.Now.AddDays(20));
    DataSet ds = new DataSet();
    ds.Tables.Add(dt);
    dsDates = ds;
}
protected void Calendar1DayRender(object sender, DayRenderEventArgs e)
{
    DateTime nextDate;
    if (dsDates != null)
    {
        foreach (DataRow dr in dsDates.Tables[0].Rows)
        {
            nextDate = (DateTime)dr["BookedDate"];
            if (nextDate.Date == e.Day.Date)
            {
                e.Day.IsSelectable = false;
                e.Cell.BackColor = System.Drawing.Color.Red;
            }
        }
    }
}
Screenshot
