Image is visible in Image Control in design time but not visible on runtime in ASP.Net

1. You add new folder as Images in your project and kept the images in that folder.

2. In solution explorer select on project right click on it then select New folder it will create new folder in your project. name it as Images.

you can add images in that folder which you want to use in project.

Add some images in that folder. by Right click on Images Folder select Add Existing Item option and then add images in your folder by selecting images.

3. After adding images in your Images Folder. set your ImageUrl as

<div class="Header">
     <asp:Image ID="image1" runat="server"
       ImageUrl="~/Images/test.jpg"  Width="500" Height="300"
       AlternateText="UNRA Fort Portal Intranet"/>
         
     </div>

It will show images at run time.