This way
<asp:ImageButton ID="ImageButton1" ImageUrl="~/TempImages/Tulips.jpg" runat="server"
ToolTip="This is a test tootltip" />
<link rel="stylesheet" href="http://qtip2.com/v/stable/jquery.qtip.css" type="text/css" />
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.3/jquery.min.js"></script>
<script type="text/javascript" src="http://qtip2.com/v/nightly/jquery.qtip.js"></script>
<script type="text/javascript">
$(function () {
$('input[title]').qtip();
});
</script>