Hi nauna,

By default the border-width is 0 and border-style is none.

So you need to set them to border-width:1px and border-style:solid.

You can combine all the border properties into one as below.

border:1px solid red

Check the below example.

HTML

<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.9.1/jquery.min.js"></script>
<script type="text/javascript" src="https://maps.googleapis.com/maps/api/js?libraries=places&sensor=false&key=AIzaSyBE1J5Pe_GZXBR_x9TXOv6TU5vtCSmEPW4"></script>
<script type="text/javascript">
    $(function () {
        $("div[class=pickup]").each(function () {
            var textbox1 = $(this).find('[id*=TextBox1]');
            var textbox2 = $(this).find('[id*=TextBox2]');
            ApplyAutoComplete(textbox1);
            ApplyAutoComplete(textbox2);
        });
    });
    function ApplyAutoComplete(input) {
        google.maps.event.addDomListener(window, 'load', function () {
            var places;
            for (var i = 0; i < input.length; i++) {
                places = new google.maps.places.Autocomplete(input[i]);
            }
            google.maps.event.addListener(places, 'place_changed', function () {
                var place = places.getPlace();
                var address = place.formatted_address;
                var mesg = "Address: " + address;
            });
        });
    }
    function Validate() {
        $("div[class=pickup]").each(function (index) {
            var textbox1 = $(this).find('[id*=TextBox1]').val();
            if (textbox1 != '') {
                var textbox2 = $(this).find('[id*=TextBox2]').val();
                if (textbox2 == '') {
                    $(this).css("border", "2px solid red");
                } else {
                    $(this).css("border", "");
                }
            } else {
                $(this).css("border", "");
            }
        });
        return false;
    }
</script>
<div>
    <asp:ListView ID="ListView1" runat="server">
        <ItemTemplate>
            <div class="pickup">
                Id :
                <asp:Label ID="Label1" runat="server" Text='<%# Eval("CustomerId") %>'></asp:Label>
                PickUp :
                <asp:TextBox ID="TextBox1" runat="server"></asp:TextBox>
                Origin :
                <asp:TextBox ID="TextBox2" runat="server"></asp:TextBox>
            </div>
        </ItemTemplate>
    </asp:ListView>
    <asp:Button Text="Save" runat="server" OnClientClick="return Validate()" />
</div>

Namespaces

C#

using System.Data;

VB.Net

Imports System.Data

Code

C#

protected void Page_Load(object sender, EventArgs e)
{
    if (!this.IsPostBack)
    {
        DataTable dt = new DataTable();
        dt.Columns.AddRange(new DataColumn[] { new DataColumn("CustomerId", typeof(int)) });
        dt.Rows.Add(1);
        dt.Rows.Add(2);
        dt.Rows.Add(3);
        this.ListView1.DataSource = dt;
        this.ListView1.DataBind();
    }
}

VB.Net

Protected Sub Page_Load(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Load
    If Not Me.IsPostBack Then
        Dim dt As DataTable = New DataTable()
        dt.Columns.AddRange(New DataColumn() {New DataColumn("CustomerId", GetType(Integer))})
        dt.Rows.Add(1)
        dt.Rows.Add(2)
        dt.Rows.Add(3)
        Me.ListView1.DataSource = dt
        Me.ListView1.DataBind()
    End If
End Sub

Screenshot